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cloning pcdna hisc mtaz addgene  (Addgene inc)


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    Addgene inc cloning pcdna hisc mtaz addgene
    Cloning Pcdna Hisc Mtaz Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+clones/pcDNA3%2E1%2FHisC-mTAZ+(Plasmid+%2331793)/pm41928626-296-163-165
    Average 91 stars, based on 3 article reviews
    cloning pcdna hisc mtaz addgene - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    CRISPR:

    Article Title: Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1
    Article Snippet: .. CRISPR/APEX-mediated proximity labeling and streptavidin pulldown—We engineered the dCas9-APEX2-NLS construct from two Addgene clones (#97421 (24) and #124617 (27)), and the expression cassette was introduced to a Zeo-pTMCS-GFP-T2A-Puro piggyBac express-ion vector (28); gRNAs against mouse Tug1 promoter region or scramble control (sequence in Supporting Table S1) were cloned into the BsaI sites of pX333 (Addgene #64073), released with XbaI-SnaB double digestion and cloned to the same piggyBac-dCas9-APEX2-NLS vector. ..

    Labeling:

    Article Title: Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1
    Article Snippet: .. CRISPR/APEX-mediated proximity labeling and streptavidin pulldown—We engineered the dCas9-APEX2-NLS construct from two Addgene clones (#97421 (24) and #124617 (27)), and the expression cassette was introduced to a Zeo-pTMCS-GFP-T2A-Puro piggyBac express-ion vector (28); gRNAs against mouse Tug1 promoter region or scramble control (sequence in Supporting Table S1) were cloned into the BsaI sites of pX333 (Addgene #64073), released with XbaI-SnaB double digestion and cloned to the same piggyBac-dCas9-APEX2-NLS vector. ..

    Construct:

    Article Title: Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1
    Article Snippet: .. CRISPR/APEX-mediated proximity labeling and streptavidin pulldown—We engineered the dCas9-APEX2-NLS construct from two Addgene clones (#97421 (24) and #124617 (27)), and the expression cassette was introduced to a Zeo-pTMCS-GFP-T2A-Puro piggyBac express-ion vector (28); gRNAs against mouse Tug1 promoter region or scramble control (sequence in Supporting Table S1) were cloned into the BsaI sites of pX333 (Addgene #64073), released with XbaI-SnaB double digestion and cloned to the same piggyBac-dCas9-APEX2-NLS vector. ..

    Article Title: Enhanced transcriptional heterogeneity mediated by NF-κB super-enhancers.
    Article Snippet: The DT40 cells were cultured in RPMI-1640 without phenol red (Wako, Japan) supplemented with 10% fetal bovine serum (Sigma-Aldrich, USA), 1% (v/v) chicken serum (Nippon Bio-test Laboratories, Japan), 75 μM 2-mercaptoethanol (Gibco, USA), 1 mM sodium pyruvate (Wako, Japan), 1% (v/v) penicillin-streptomycin solution (Nacalai Tesque, Japan), 1% (v/v) 100x MEM non-essential amino acids solution (Wako), and 2 mM L-glutamine (Nacalai Tesque). .. The cells were cultured at 39 ̊C and 5% CO2 in a humidified incubator. mKate2-BRD4S transposon plasmid construction We engineered the PB-TA-ERP2-mKate2-BRD4S construct from two addgene clones (65378 [55] and 80477 [56]) and pmKate2-H2B (Evrogen, Russia). ..

    Clone Assay:

    Article Title: Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1
    Article Snippet: .. CRISPR/APEX-mediated proximity labeling and streptavidin pulldown—We engineered the dCas9-APEX2-NLS construct from two Addgene clones (#97421 (24) and #124617 (27)), and the expression cassette was introduced to a Zeo-pTMCS-GFP-T2A-Puro piggyBac express-ion vector (28); gRNAs against mouse Tug1 promoter region or scramble control (sequence in Supporting Table S1) were cloned into the BsaI sites of pX333 (Addgene #64073), released with XbaI-SnaB double digestion and cloned to the same piggyBac-dCas9-APEX2-NLS vector. ..

    Article Title: Enhanced transcriptional heterogeneity mediated by NF-κB super-enhancers.
    Article Snippet: The DT40 cells were cultured in RPMI-1640 without phenol red (Wako, Japan) supplemented with 10% fetal bovine serum (Sigma-Aldrich, USA), 1% (v/v) chicken serum (Nippon Bio-test Laboratories, Japan), 75 μM 2-mercaptoethanol (Gibco, USA), 1 mM sodium pyruvate (Wako, Japan), 1% (v/v) penicillin-streptomycin solution (Nacalai Tesque, Japan), 1% (v/v) 100x MEM non-essential amino acids solution (Wako), and 2 mM L-glutamine (Nacalai Tesque). .. The cells were cultured at 39 ̊C and 5% CO2 in a humidified incubator. mKate2-BRD4S transposon plasmid construction We engineered the PB-TA-ERP2-mKate2-BRD4S construct from two addgene clones (65378 [55] and 80477 [56]) and pmKate2-H2B (Evrogen, Russia). ..

    Article Title: Noncanonical roles of ATG5 and membrane atg8ylation in retromer assembly and function
    Article Snippet: Plasmids used in this study, such as ATG5 were generated by first cloning inserts into pDONR221 (Gateway Technology cloning vector, Thermo Scientfic) using a BP cloning reaction and the expression vectors were made utilizing LR cloning reaction (Gateway, Thermo Fisher) in appropriate (pDEST) destination vectors for immunoprecipitation assay. .. Addgene clones were: eGFP-Rab7 WT (Addgene, #12605), eGFP-Rab7 Q67L (Addgene, #28049), and eGFP-Rab7 T22N (Addgene, #28049). ..

    Article Title: Noncanonical roles of ATG5 and membrane atg8ylation in retromer assembly and function
    Article Snippet: Plasmids used in this study, such as ATG5 were generated by first cloning inserts into pDONR221 (Gateway Technology cloning vector, Thermo Scientfic) using a BP cloning reaction and the expression vectors were made utilizing LR cloning reaction (Gateway, Thermo Fisher) in appropriate (pDEST) destination vectors for immunoprecipitation assay. .. Addgene clones were: eGFP- Rab7 WT (Addgene, #12605), eGFP- Rab7Q67L (Addgene, #28049), and eGFP- Rab7T22N (Addgene, #28049). ..

    Expressing:

    Article Title: Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1
    Article Snippet: .. CRISPR/APEX-mediated proximity labeling and streptavidin pulldown—We engineered the dCas9-APEX2-NLS construct from two Addgene clones (#97421 (24) and #124617 (27)), and the expression cassette was introduced to a Zeo-pTMCS-GFP-T2A-Puro piggyBac express-ion vector (28); gRNAs against mouse Tug1 promoter region or scramble control (sequence in Supporting Table S1) were cloned into the BsaI sites of pX333 (Addgene #64073), released with XbaI-SnaB double digestion and cloned to the same piggyBac-dCas9-APEX2-NLS vector. ..

    Control:

    Article Title: Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1
    Article Snippet: .. CRISPR/APEX-mediated proximity labeling and streptavidin pulldown—We engineered the dCas9-APEX2-NLS construct from two Addgene clones (#97421 (24) and #124617 (27)), and the expression cassette was introduced to a Zeo-pTMCS-GFP-T2A-Puro piggyBac express-ion vector (28); gRNAs against mouse Tug1 promoter region or scramble control (sequence in Supporting Table S1) were cloned into the BsaI sites of pX333 (Addgene #64073), released with XbaI-SnaB double digestion and cloned to the same piggyBac-dCas9-APEX2-NLS vector. ..

    Sequencing:

    Article Title: Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1
    Article Snippet: .. CRISPR/APEX-mediated proximity labeling and streptavidin pulldown—We engineered the dCas9-APEX2-NLS construct from two Addgene clones (#97421 (24) and #124617 (27)), and the expression cassette was introduced to a Zeo-pTMCS-GFP-T2A-Puro piggyBac express-ion vector (28); gRNAs against mouse Tug1 promoter region or scramble control (sequence in Supporting Table S1) were cloned into the BsaI sites of pX333 (Addgene #64073), released with XbaI-SnaB double digestion and cloned to the same piggyBac-dCas9-APEX2-NLS vector. ..

    Cell Culture:

    Article Title: Enhanced transcriptional heterogeneity mediated by NF-κB super-enhancers.
    Article Snippet: The DT40 cells were cultured in RPMI-1640 without phenol red (Wako, Japan) supplemented with 10% fetal bovine serum (Sigma-Aldrich, USA), 1% (v/v) chicken serum (Nippon Bio-test Laboratories, Japan), 75 μM 2-mercaptoethanol (Gibco, USA), 1 mM sodium pyruvate (Wako, Japan), 1% (v/v) penicillin-streptomycin solution (Nacalai Tesque, Japan), 1% (v/v) 100x MEM non-essential amino acids solution (Wako), and 2 mM L-glutamine (Nacalai Tesque). .. The cells were cultured at 39 ̊C and 5% CO2 in a humidified incubator. mKate2-BRD4S transposon plasmid construction We engineered the PB-TA-ERP2-mKate2-BRD4S construct from two addgene clones (65378 [55] and 80477 [56]) and pmKate2-H2B (Evrogen, Russia). ..

    Plasmid Preparation:

    Article Title: Enhanced transcriptional heterogeneity mediated by NF-κB super-enhancers.
    Article Snippet: The DT40 cells were cultured in RPMI-1640 without phenol red (Wako, Japan) supplemented with 10% fetal bovine serum (Sigma-Aldrich, USA), 1% (v/v) chicken serum (Nippon Bio-test Laboratories, Japan), 75 μM 2-mercaptoethanol (Gibco, USA), 1 mM sodium pyruvate (Wako, Japan), 1% (v/v) penicillin-streptomycin solution (Nacalai Tesque, Japan), 1% (v/v) 100x MEM non-essential amino acids solution (Wako), and 2 mM L-glutamine (Nacalai Tesque). .. The cells were cultured at 39 ̊C and 5% CO2 in a humidified incubator. mKate2-BRD4S transposon plasmid construction We engineered the PB-TA-ERP2-mKate2-BRD4S construct from two addgene clones (65378 [55] and 80477 [56]) and pmKate2-H2B (Evrogen, Russia). ..



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